Formation of Non-spherical Hydrogel Microstructures Using Non-equilibrium Aqueous Two-phase Systems
نویسندگان
چکیده
We propose a unique process to generate non-spherical hydrogel microstructures using an aqueous two-phase system (ATPS) in a non-equilibrium state. A dextran (Dex) solution containing cells and hydrogel precursor (sodium alginate; NaA) was introduced into microfabricated chambers, which was gradually shrunk and deformed after pouring a polyethylene glycol (PEG) solution. Following the addition of a gelation agent, we successfully obtained non-spherical (toroidal or cup-shaped) hydrogel microstructures with concentrated cells. We confirmed that the microchamber geometries, compositions of the solutions, and the surface wettability dominate the hydrogel morphologies.
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